Effect of Chlorhexidine and Fluoride Varnishes on Streptococcus mutans Count and Dental Plaque Index

Autores

  • Fernando Yamamoto Chiba São Paulo State University, School of Dentistry, Araçatuba, Department of Child and Social Dentistry, SP, Brazil.
  • Cléa Adas Saliba Garbin São Paulo State University, School of Dentistry, Araçatuba, Department of Child and Social Dentistry, SP, Brazil.
  • Tânia Adas Saliba São Paulo State University, School of Dentistry, Araçatuba, Department of Child and Social Dentistry, SP, Brazil.
  • Artênio José Ísper Garbin São Paulo State University, School of Dentistry, Araçatuba, Department of Child and Social Dentistry, SP, Brazil.
  • Suzely Adas Saliba Moimaz São Paulo State University, School of Dentistry, Araçatuba, Department of Child and Social Dentistry, SP, Brazil.

DOI:

https://doi.org/10.17921/2447-8938.2019v21n4p371-5

Resumo

Abstract

The control of dental plaque by chemical means can be a valuable auxiliary resource for individuals who have difficulties in performing dental plaque control through regular oral hygiene procedures. The aim of this study was to evaluate the effect of chlorhexidine and fluorine varnishes on the levels of Streptococcus mutans (S. mutans) present in the dental biofilm and on the dental plaque index. The study was carried out in a sample comprised thirty-nine schoolchildren between 13 and 15 years of age, distributed into 2 groups. Group I received a single layer of varnish with chlorhexidine and thymol on the maxillary right first molar. Group II received the application of varnish with fluoride following the same methodology. S. mutans count and dental plaque index recording were performed 7 days before the application and 7, 15 and 30 days after the treatment. It was observed that chlorhexidine and fluoride varnishes significantly reduced the number of S. mutans in dental plaque for 30 days (p<0.05). There was no difference between the varnishes. With regard to the dental plaque index, there was no alteration in any of the experimental time periods for both groups. It was concluded that varnishes, mainly those that contain fluoride, can be an interesting alternative for the prevention of dental caries in collective oral health programs.

 

Keywords: Chlorhexidine. Fluorides. Dental Plaque.

 

Resumo

O controle da placa dental por meios químicos pode ser um valioso recurso auxiliar para indivíduos que apresentam dificuldades em realizar o controle da placa dental por meio de procedimentos regulares de higiene bucal. O objetivo neste estudo foi avaliar o efeito dos vernizes de clorexidina e de flúor sobre os níveis de Streptococcus mutans (S. mutans) presentes no biofilme dental e sobre o índice de placa dental. O estudo foi realizado em uma amostra composta por 39 escolares, entre 13 e 15 anos de idade, distribuídos em 2 grupos. O grupo I recebeu uma única aplicação de verniz com clorexidina e timol no primeiro molar superior direito. O grupo II recebeu a aplicação de verniz com flúor seguindo a mesma metodologia. A contagem de S. mutans e o índice de placa dental foram registrados 7 dias antes da aplicação e 7, 15 e 30 dias após o tratamento. Observou-se que os vernizes de clorexidina e flúor reduziram significativamente o número de S. mutans na placa dental por 30 dias (p <0,05). Não houve diferença entre os vernizes. Com relação ao índice de placa dental, não houve alteração em nenhum dos períodos experimentais para ambos os grupos. Concluiu-se que os vernizes, principalmente aqueles que contêm flúor, podem ser uma alternativa interessante para a prevenção da cárie dentária em programas coletivos de saúde bucal.

 

Palavras-chave: Clorexidina. Fluoretos. Placa Dentária.

Biografia do Autor

Fernando Yamamoto Chiba, São Paulo State University, School of Dentistry, Araçatuba, Department of Child and Social Dentistry, SP, Brazil.

Department of Child and Social Dentistry

Cléa Adas Saliba Garbin, São Paulo State University, School of Dentistry, Araçatuba, Department of Child and Social Dentistry, SP, Brazil.

Department of Child and Social Dentistry

Tânia Adas Saliba, São Paulo State University, School of Dentistry, Araçatuba, Department of Child and Social Dentistry, SP, Brazil.

Department of Child and Social Dentistry

Artênio José Ísper Garbin, São Paulo State University, School of Dentistry, Araçatuba, Department of Child and Social Dentistry, SP, Brazil.

Department of Child and Social Dentistry

Suzely Adas Saliba Moimaz, São Paulo State University, School of Dentistry, Araçatuba, Department of Child and Social Dentistry, SP, Brazil.

Department of Child and Social Dentistry

Referências

Rode SM, Gimenez X, Montoya VC, Gómez M, Blanc SL, Medina M, Salinas E, et al. Daily biofilm control and oral health: consensus on the epidemiological challenge-Latin American Advisory Panel. Braz Oral Res 2012;26 Suppl 1:133-43. doi: 10.1590/S1806-83242012000700020

Sharma M, Pandit IK, Srivastava N, Gugnani N, Gupta M. A comparative evaluation of efficacy of Streptococcus mutans counts in saliva: an in vivo study. Int J Clin Pediatr Dent 2018;11(2):94-9. doi: 10.5005/jp-journals-10005-1492.

Sudhanthar S, Lapinski J, Turner J, Gold J, Sigal Y, Thakur K, et al. Improving oral health through dental fluoride varnish application in a primary care paediatric practice. BMJ Open Qual 2019;8(2):e000589. doi: 10.1136/bmjoq-2018-000589.

O'Mullane DM, Baez RJ, Jones S, Lennon MA, Petersen PE, Rugg-Gunn AJ, et al. Fluoride and oral health. Community Dent Health 2016;33(2):69-99. doi: 10.1922/CDH_3707O’Mullane31.

Scherrer CR, Naavaal S. Cost-Savings of Fluoride Varnish Application in Primary Care for Medicaid-Enrolled Children in Virginia. J Pediatr 2019; pii: S0022-3476(19)30592-X. doi: 10.1016/j.jpeds.2019.05.026.

Ben Khadra GM, Arrag EA, Alammori M, AlKadi MF. The effect of chlorhexidine-thymol and fluoride varnishes on the levels of Streptococcus mutans in saliva in children aged 6-8 years. Indian J Dent Res 2019;30(1):67-72. doi: 10.4103/ijdr.IJDR_208_17.

Figuero E, Nóbrega DF, García-Gargallo M, Tenuta LM, Herrera D, Carvalho JC. Mechanical and chemical plaque control in the simultaneous management of gingivitis and caries: a systematic review. J Clin Periodontol 2017;44 Suppl 18:S116-S134. doi: 10.1111/jcpe.12674.

Balagopal S, Arjunkumar R. Chlorhexidine: The gold standard antiplaque agent. Pharm Sci & Res 2013;12:270-274.

Carvalho DM, Salazar M, Oliveira BH, Coutinho ES. Fluoride varnishes and decrease in caries incidence in preschool children: a systematic review. Rev Bras Epidemiol 2010;13(1):139-49. doi: 10.1590/S1415-790X2010000100013

Naidu S, Tandon S, Nayak R, Ratnanag PV, Prajapati D, Kamath N. Efficacy of concomitant therapy with fluoride and chlorhexidine varnish on remineralization of incipient lesions in young children. Int J Clin Pediatr Dent 2016;9(4):296-302. doi: 10.5005/jp-journals-10005-1381.

Mishra P, Fareed N, Battur H, Khanagar S, Bhat MA, Palaniswamy J. Role of fluoride varnish in preventing early childhood caries: A systematic review. Dent Res J (Isfahan) 2017;14(3):169-76. doi: 10.4103/1735-3327.208766.

Arruda AO, Senthamarai Kannan R, Inglehart MR, Rezende CT, Sohn W. Effect of 5% fluoride varnish application on caries among school children in rural Brazil: a randomized controlled trial. Community Dent Oral Epidemiol 2012;40(3):267-76. doi: 10.1111/j.1600-0528.2011.00656.x.

Petersson LG. The role of fluoride in the preventive management of dentin hypersensitivity and root caries. Clinical Oral Investigations 2013;17(Suppl 1):63-71. doi: 10.1007/s00784-012-0916-9.

Slot DE, Vaandrager NC, Van Loveren C, Van Palenstein Helderman WH, Van der Weijden GA. The effect of chlorhexidine varnish on root caries: a systematic review. Caries Res 2011;45(2):162-73. doi: 10.1159/000327374.

Ainamo J, Bay I. Problems and proposals for recording gingivitis and plaque. Int Dent J 1975;25:229-35.

Gold OG, Jordan HV, Van Houte J. A selective medium for Streptococcus mutans. Arch Oral Biol 1973;18(11):1357-64.

Van Rijkom HM, Truin GJ, vant’t Hof MA. A meta-analysis of clinical atudies on the caries-inibiting effect of cholorhexidine treatment. J Dent Res 1996;5:790-5. doi: 10.1177/00220345960750020901.

Tyski S, Bocian E, Mikucka A, Grzybowska W. Antibacterial activity of selected commercial products for mouth washing and disinfection, assessed in accordance with PN-EN 1040. Med Sci Monit 2013;19:458-66. doi: 10.12659/MSM.883952.

Badjatia S, Badjatia RG, Thanveer K, Krishnan ACG. Effects of fluoride varnish on Streptococcus mutans count in saliva. Int J Clin Pediatr Dent 2017;10(1):62-6. doi: 10.5005/jp-journals-10005-1409.

George AM, Kalangi SK, Vasudevan M, Krishnaswamy NR. Chlorhexidine varnishes effectively inhibit Porphyromonas gingivalis and Streptococcus mutans: an in vivo study. J Indian Soc Periodontol 2010;14(3):178-80. doi: 10.4103/0972-124X.75913.

Okada EM, Ribeiro LN, Stuani MB, Borsatto MC, Fidalgo TK, Paula-Silva FW, et al. Effects of chlorhexidine varnish on caries during orthodontic treatment: a systematic review and meta-analysis. Braz Oral Res 2016;30(1):e115. doi: 10.1590/1807-3107BOR-2016.vol30.0115.

Baygin O, Tuzuner T, Kusgoz A, Senel AC, Tanriver M, Arslan I. Antibacterial effects of fluoride varnish compared with chlorhexidine plus fluoride in disabled children. Oral Health Prev Dent 2014;12(4):373-82. doi: 10.3290/j.ohpd.a32129.

Ekenback SB, Linder LE, Lonnies H. Effect of four dental varnishes on the colonization of cariogenic bacteria on exposed sound root surfaces. Caries Res 2000;34(1):70-4. doi: 10.1159/000016572.

Zickert I, Emilson CG. Effect of a fluoride-containing varnish on Streptococcus mutans in plaque and saliva. Scand J Dent Res 1982;90(6):423-8.

Wallman C, Birkhed D. Effect of chlorhexidine varnish and gel on mutans streptococci in margins of restorations in adults. Caries Res 2002;36(5):360-5. doi: 10.1159/000065957.

Sandham JH, Nadeau L, Phillips HI. The effect of chlorhexidine varnish treatment on salivary mutans streptococcal levels in child orthodontic patients. J Dent Res 1992;71:32-5. doi: 10.1177/00220345920710010501.

Ribeiro LGM, Hashizume LN, Maltz M. Effect of different 1% chlorhexidine varnish regimens on levels of mutans streptococci in saliva and dental biofilm. Am J Dent 2008;21:295-9.

Amorim RG, Leal SC, Bezerra AC, Amorim FP, Toledo OA. Association of chlorhexidine and fluoride for plaque control and white spot lesion remineralization in primary dentition. Int J Paediatr Dent 2008;18(6):446-51. doi: 10.1111/j.1365-263X.2008.00914.x.

Weiger R, Friedrich C, Netuschil L, Schlagenhauf U. Effect of chlorhexidine-containing varnish (Cervitec) on microbial vitality and accumulation of supragingival dental plaque in humans. Caries Res 1994;28(4):267-71. doi: 10.1159/000261984.

Mohammadi TM, Hajizamani A, Hajizamani HR, Abolghasemi B. Fluoride varnish effect on preventing dental caries in a sample of 3-6 years old children. J Int Oral Health 2015;7(1):30-5.

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Publicado

2019-12-20

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1.
Chiba FY, Garbin CAS, Saliba TA, Garbin AJ Ísper, Moimaz SAS. Effect of Chlorhexidine and Fluoride Varnishes on Streptococcus mutans Count and Dental Plaque Index. J. Health Sci. [Internet]. 20º de dezembro de 2019 [citado 29º de março de 2024];21(4):371-5. Disponível em: https://journalhealthscience.pgsscogna.com.br/JHealthSci/article/view/7113

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